Welcome to MOSAS

Get Started

Step 1

First, add sequences to your database. You can upload a FASTA file, or query GenBank for sequences.

If you're using MOSAS to annotate a sequence:

Step 2

check out the sequence detail page and select a sequence

Step 3

use the scan tool to populate the feature table with automatic estimates

Step 4

click on the visualize button to examine tRNAs for accuracy.
commit the good ones
delete the bad ones.

Step 5

delete any erroneous ORFs (usually they are smaller and have lower BLAST scores).

Step 6

click on each protein-coding feature to bring up the feature detail page. choose the correct start and stop locations by clicking on the sequence

If you're using MOSAS to organize data:

Step 2

construct some datasets. for lots of datasets where the sequence names will contain the name of the dataset in which they belong, you can use the dataset generator

Step 3

use the filter to add sequences to your datasets on the dataset detail page.

Step 4

if desired, use alignment or statistic tools at the bottom of the dataset detail page.

Step 5

download the organized sets or alignment results for local analysis

Features at-a-glance

• store sequences: each user may store up to 2,000 nucleotide or amino acid sequences on our server.
• retrieve sequences: you can query GenBank for direct input into your personal database.
• define sequence sets: search filters will help you sort your sequences by gene or organism.
• run analytical tools: MOSAS provides a web interface to command-line tools like INFERNAL or MUSCLE.
• annotate sequences: use tRNAscan-SE, an ORF finder, and BLAST for automatic annotation.
• download results: in 10+ formats via readseq.